Family and friends of Alyson Porter, many of them keen biker colleagues who support the Christian Bikers Breakfast she founded at Knowle Green Congregational Church, attended the service and took part in a procession from Derby Road.
The cortege included a three-wheeler trike hearse, specially built for Alyson, 47.
Before the funeral, her husband Dave said: "She meant a lot to so many and we want to give her the rousing send-off she would love. I'm expecting lots of biker friends from all over the country."
Alyson, who had fought cancer since a first diagnosis when she was 28, had managed to test drive the trike.
She saw it for a final time a few days before she died, when CBB bikers drove it to Longridge Community Hospital and she waved from her ward window.
Born and educated in Swinton, Salford, Alyson triend as a nurse at Trafford General Hospital before becoming matron of a nursing home and then a laser nurse at a private clinic in Bolton.
She is survived by her husband, two daughters, a stepson, her parents Alan and Jean Carter, and her sister, Helen.